Image analysis

Our laboratory possesses unique equipment and expertise for the analysis of nervous system diseases using advanced microscopic imaging techniques.

Team

Scientific manager. Marc Dhenain

Image Acquisition

In order to acquire histological data images, our platform is equipped with:

Confocal microscopes
Optical microscopes
° A fluorescence microscope (Olympus XI)
° A Leica fluorescence microscope

Slide scanners
° A Leica Aperio Versa slide scanner
° A low-resolution slide scanner

Image Analyses

This platform performs qualitative and quantitative analyses of microscopic data from human tissues and pathology models and therapies developed in the laboratory.

Activities

° Synaptic quantification in neurodegenerative diseases
° Structural analyses of cells (Sholl analysis, 3D reconstruction)
° Time-lapse confocal image analysis to quantify Schwann cell migration

Equipments 

° Work stations for image analysis

° Wacom tablets for region of interest segmentation

° Different softwares are used for tasks such as acquiring mosaic images, automated object detection by thresholding, stereological cell counting, or three-dimensional image analysis.

Access

These facilities are available to external users upon request, generally within the framework of scientific collaboration. Submitted projects are evaluated by a scientific committee composed of experts in various imaging technologies.

Contact: Marc.Dhenain@inserm.fr